Business Research Methodologies Assignment Example | Topics and Well Written Essays - 1500 words

Business Research Methodologies - Assignment Example The world of banking finance is highly integrated with various kinds of complex concepts as well as various kinds of frameworks related to operating standards as well as legal issues. The integration of technology in its complex processes will no doubt help in better value generation for the customers while providing a level ground in regards to the competing of the financial organizations with each other. In this project, the focus is on gauging the impacts on the customers of Lloyd’s Bank with regards to growing general fascination towards internet banking. The research proposal for this topic comprises an introduction, a brief literature review as well as the method for conducting the research. Introduction The world of the 21st century promotes an environment where tremendous competition is a steady factor of everyday life of the masses around the world. The competition factor has become a constant issue in the modern day society mainly because of rapid technological chang es, which is coupled with fast paced development. The technological boom that has happened since the early part of the previous decade has brought into play a strong wave of technological enrichment of the lives of the masses in the developing as well as the developed countries. The technological boom of the early 2000s enrolled into effect tremendous amount of technological progress on the lines of telecommunication technology as well as the rapid acceptance and penetration of the internet. The high level of penetration of the internet has played a major role in creating a seamless virtual world in the internet domain, where there is no presence physical geographical boundaries. The virtual world which is connected through the internet platform has merged the various nations and countries around the world into a single entity. This has greatly facilitated the rise of various kinds of trends related to globalization and innovation. It has to be mentioned that the trends of innovatio n greatly facilitates in the process of new product development in various countries around the world. Interestingly, the virtual medium of the internet plays the role of communicating the value of the newly developed innovative products to the customers and target audience around the world thereby creating a typical trend of globalization. This trend of globalization, which might emerge from a market that can be either located in the developed or the developing region might result in the creation of demand for new kinds of products and services in various markets around the world (Doole and Lowe, 2008, p. 3). Sensing the demand for new kinds of products and services in various global markets, various organizations around the world are focusing on the process of market development. As a method of market development, the organizations which are based in a particular market are increasingly participating in entering in to foreign markets around the world. As per the current trend, due to the slowdown that is persisting in the economies of various developed markets present in the western nations of UK, Europe and USA, companies are increasingly focusing on new emerging markets of the BRIC nations. Thus, it has to be said that the development of business ventures of various international organizations is creating the need of highly dynamic

Leadership Styles In Professional Nursing Research Paper

Leadership Styles In Professional Nursing - Research Paper Example This component of the essay seeks to achieve two major aims. Firstly, it is to describe two different leadership styles in the nursing profession and also discuss how these two nursing leadership styles can be implemented in a professional nursing practice. In the first place, Rampur (2011) identifies two major leadership styles in the nursing profession. According to him, these leadership styles are democratic and autocratic leadership styles. Earlier in his submission, Rampur (2011) elucidates that leaders in the nursing profession are mostly seen to be either nurse managers who are â€Å"assigned the obligation of handling one unit† or nurse executives who are â€Å"responsible for the operations of all in-patient nursing units.† Under whatever portfolio a nurse might be in a leadership position, the leadership styles mentioned earlier happen to reign at one point in time or the other. On the whole, the type of leadership style that implemented should depend on a spe cific situation rather than adopting one of the two as a routine leadership style (Cummings, 2010). With the democratic leadership style, the nurse leader â€Å"engages his nurses in decision-making and let them carry out their work in an independent manner (Rampur, 2011).† In the autocratic nursing leadership however, the nurse leader â€Å"would provide instructions without looking for inputs and superintend his nurses in a close manner (Rampur, 2011).† Simply put, democratic leadership allows for group participation, discussion and contribution as far as decision making is concerned but with autocratic leadership style, subordinates always have to take instructions from the leaders. Without passing outright judgment on the either types of nursing leadership styles as to which is right and which is wrong, it is worth noting that there are different ways and at different points in time that the various leadership styles are brought to work. As a matter of fact, the is sue of whether a particular leadership style is right or wrong would come in if the right leadership style is implemented at the wrong time and vice versa. In implementing any of the leadership styles, it is important that leaders put into consideration the â€Å"health authority structures, inter-professional teamwork and conflict, the actions and decisions of senior administrators and government officials, changing public demographics and consumer expectations of the healthcare system, and the implementation of new knowledge, evidence and innovation†

Mathematical Golden Ratio in Nature, Music and Sex Essay

Mathematical Golden Ratio in Nature, Music and Sex - Essay Example The essay "Mathematical Golden Ratio in Nature, Music, and Sex" analyzes the issue of how the mathematical golden ratio is used in nature, music, and sex appeal. The golden ratio is developed by from the extreme and mean ratio rule that states that â€Å"a straight line is said to have been cut in extreme and mean ratio when, as the whole line is to the greater segment, the greater to the lesser†. The ratio of the straight line’s unequal segments is the origin of several natural scenes of mathematics interaction with nature. Only a handful of retrogressive and conservative theorists have risen to offer sensible criticism to the best explanation to mathematical infusion to nature’s inseparable interaction with mathematical theory. Perhaps the best brain that this age has experienced was possessed by Einstein who devoted much of his intense academic work to find out on the natures engagement with mathematics. According to Livio, several natural arrangements remain strictly controlled by the ratio such as the patterns seen in leaf structures and arrangement of the branches (phyllotaxis), seed arrangement patterns on plants as well as the formation of natural crystals. The Fibonacci sequence explains the patterns employed in botanical systems which depict market design and order that no other discipline elaborates on but mathematics. In light of these phenomena, mathematics fascinating facts in nature as dictated by the golden ratio seems to be the best explanation behind nature’s orderliness.

Leadership Styles In Professional Nursing Research Paper

Leadership Styles In Professional Nursing - Research Paper Example This component of the essay seeks to achieve two major aims. Firstly, it is to describe two different leadership styles in the nursing profession and also discuss how these two nursing leadership styles can be implemented in a professional nursing practice. In the first place, Rampur (2011) identifies two major leadership styles in the nursing profession. According to him, these leadership styles are democratic and autocratic leadership styles. Earlier in his submission, Rampur (2011) elucidates that leaders in the nursing profession are mostly seen to be either nurse managers who are â€Å"assigned the obligation of handling one unit† or nurse executives who are â€Å"responsible for the operations of all in-patient nursing units.† Under whatever portfolio a nurse might be in a leadership position, the leadership styles mentioned earlier happen to reign at one point in time or the other. On the whole, the type of leadership style that implemented should depend on a spe cific situation rather than adopting one of the two as a routine leadership style (Cummings, 2010). With the democratic leadership style, the nurse leader â€Å"engages his nurses in decision-making and let them carry out their work in an independent manner (Rampur, 2011).† In the autocratic nursing leadership however, the nurse leader â€Å"would provide instructions without looking for inputs and superintend his nurses in a close manner (Rampur, 2011).† Simply put, democratic leadership allows for group participation, discussion and contribution as far as decision making is concerned but with autocratic leadership style, subordinates always have to take instructions from the leaders. Without passing outright judgment on the either types of nursing leadership styles as to which is right and which is wrong, it is worth noting that there are different ways and at different points in time that the various leadership styles are brought to work. As a matter of fact, the is sue of whether a particular leadership style is right or wrong would come in if the right leadership style is implemented at the wrong time and vice versa. In implementing any of the leadership styles, it is important that leaders put into consideration the â€Å"health authority structures, inter-professional teamwork and conflict, the actions and decisions of senior administrators and government officials, changing public demographics and consumer expectations of the healthcare system, and the implementation of new knowledge, evidence and innovation†

The Acquisition of Tense by Arabic Learners of English Language Thesis Proposal

The Acquisition of Tense by Arabic Learners of English Language - Thesis Proposal Example This proposal was motivated by an interest in the subject as well as the knowledge that the results of the study are potentially productive and could provide necessary knowledge in the area of linguistic science. This subject is a problem of significance for the students it affects and the knowledge to be gained from this study stands to benefit more than the population of peoples with Arabic as a first language. It is my hope that the findings of this study will also be used to assist other L2 students, and perhaps enrich their relationship with a second language. Among the problems faced by English students with Arabic as their first language is the acquisition of tenses. The morphemes of the English language differ greatly from those of Arabic, and the irregularities of tenses must be memorized, making the acquisition of tenses one of the most difficult parts of the learning process. As stated by Johanne Paradis, this situation â€Å"†¦is a vulnerable domain for English lang uage learners across acquisition contexts†¦Ã¢â‚¬  Since one of two allomorphs pertaining to the past tense is possible, either [td] or [t or d], the English past tense exhibits a quasi-regularity. These combined attributes create difficulties for the would-be English learner. To appreciate the hardship of the L2 student, data will be collected from thirty students at three consecutive levels of ability. Two approaches will be taken while analyzing the data in an attempt to prove two hypotheses.... Since one of two allomorphs pertaining to the past tense is possible, either [td] or [t or d], the English past tense exhibits a quasi-regularity (McClelland). These combined attributes create difficulties for the would-be English learner. To appreciate the hardship of the L2 student, data will be collected from thirty students at three consecutive levels of ability. Two approaches will be taken while analyzing the data in an attempt to prove two hypotheses. Problem Statement: Learning difficulties arising for L2 students can affect certain aspects of their lives. In the case of foreign students, attempts to integrate into a new country can be inhibited by linguistic difficulties. "knowing a language involves mastering an intricate system full of surprising regularities and idiosyncrasies." (Sag and Wasow, 2001) For people attempting to learn English as a second language, it can be embarrassing and often debilitating to incorrectly express tenses. In the workplace, people with English as a second language are often misjudged as unintelligent. Added to the difficulties most adults have with the correct acquisition of phonological sounds, (Escudero, 2005) the learning of English for Arabic speakers is quite a challenge. The confusion of past tenses was one of the major errors in tenses reported in a study of Arab learners of English at the University of Sudan (Kambal, 1980). Therefore, a study concerning the acquisition of English tenses by Arabic students can assist students with similar problems by determining the most effective solution. Hypotheses: There are two hypotheses which will be used to attempt an explanation of the difficulties that

Survey on Chocolate Essay Example for Free

Survey on Chocolate Essay A delicate tree, cacao is only grown in rain forests in the tropics, usually on large plantations, where it must be protected from wind and intense sunlight. The tree is harvested twice a year. Milk chocolate was invented in 1876 by a Swiss chocolatier, Daniel Peter (1836-1919) of Vevey, Geneva. Daniel Peter successfully combined chocolate with powdered milk to produce the first milk chocolate. Today, the finest chocolate is still made in Switzerland, and the consumption of milk chocolate far outweighs that of plain chocolate. Chocolate was introduced to the United States in 1765 when John Hanan brought cocoa beans from the West Indies into Dorchester, Massachusetts, to refine them with the help of Dr. James Baker. The first chocolate factory in the country was established there. †¢ Chocolate Glossary Unsweetened Chocolate: It is also called baking, plain or bitter chocolate. Since no sugar has been added to the chocolate it has a strong, bitter taste that is used in cooking and baking but is never eaten out of hand. Bittersweet Chocolate: Still dark, but a little sweeter than unsweetened. It is unsweetened chocolate to which sugar, more cocoa butter, lecithin, and vanilla has been added. It has less sugar and more liquor than semisweet chocolate but the two are interchangeable in baking. Bittersweet has become the sophisticated choice of chefs. It contains a high percentage (up to 75%) of cocoa solids, and little (or no) added sugar. Semisweet Chocolate: Slightly sweetened during processing, and most often used in frostings, sauces, fillings, and mousses. They are interchangeable in most recipes. The favorite of most home bakers. It contains a high percentage (up to 75%) of cocoa solids, and little (or no) added sugar. German Chocolate: Dark, but sweeter than semisweet. German chocolate is the predecessor to bittersweet. It has no connection to Germany; it was developed by a man named German. Milk Chocolate or Sweet Chocolate: Candy bar chocolate. Chocolate to which whole and/or skim milk powder has been added. Rarely used in cooking because the protein in the added milk solids interferes with the texture of the baked products. It contains approximately 20 percent cocoa solids. White Chocolate: Many people might argue that white chocolate is not really chocolate. It is made from sweetened cocoa butter mixed with milk solids, sometimes with vanilla added. Since cocoa butter is derived from the cocoa bean, then we can only conclude that real white chocolate is indeed chocolate. Conveture: A term generally used to describe high-quality chocolate used by professional bakers in confectionery and baked products. The word means to cover or to coat. It has more cocoa butter than regular chocolate. Its specially formulated for dipping and coating things like truffles. Chocolate of this quality is often compared to tasting fine wine because subtleties in taste are often apparent, especially when you taste a variety of semisweet and bittersweet couvertures with different percentages of sugar and chocolate liquor. †¢ How Chocolate Is Made Cacao trees are often interplanted with tall shade trees to protect them from direct sunlight. Pods grow on the trunks and larger branches of the trees and take five to six months to ripen. Fruit on the higher branches are harvested with blades on long handles and lower branches are cut with machetes. The pods are cut open with machetes to reveal between 20 to 40 beans each, surrounded by a mass of stickly, white pulp. Traditionally, this was done immediately after harvest; today, pods are sometimes first stored whole for a few days to prime them for fermentation. Fermenting begins when the beans come into contact with the air. Here, a workrt uses a stick to gauge the depth of the mass in a vara, or measuring box, to determine the wage of the harvester, before transferring it to the fermentation bin. During fermentation, the pulp disintegrates, producing steamy heat and a pervasive, yeasty, sour smell. It is at this point that the beans first develop thier complex characteristics. Drying of the beans after fermentation is done on slatted wooden trays in the open air. The beans are spread out evenly and raked periodically so that they dry uniformly. As the beans dry, their colors deepen, turning them into a carpet of sepia, umber, and mocha. Aeration of the dried beans during storage is important to prevent the formation of mold. A worker tosses beans with a shovel to expose them evenly to the air. Grading of the beans is done mechanically at the larger farms; smaller producers do it by hand. From baskets, the dried beans are transferred to burlap bags and transported to local selling stations, where they may be bought by large companies for export. Arriving at the chocolate mills, the beans undergo a thorough cleaning, followed by the roasting which brings out the particular flavor of each variety. Throughout this process, a constant and exact temperature must be maintained. Correct roasting is exceedingly important since under-roasting leaves a raw taste and over-roasting results in a high pungent or even burnt flavor. Now comes the cooling, shelling, and winnowing, from which the cocoa beans emerge cleaned and ready for blending. This important process requires expert knowledge and skill. Not only must the beans be selected which will produce the best chocolate flavor, but uniformity of blend must be preserved year in and year out. After the blending, the cocoa beans are milled or slowly ground between great heated millstones. Under heat and tremendous pressure, the cocoa butter melts and mixes with other parts of the beans forming the ruddy chocolate liquor. The fragrant chocolate odor is now noticeable. The liquor is then treated according to the product to be made. For unsweetened chocolate, the liquor is poured into molds and cooled rapidly in refrigerating rooms. Then the cacao emeres in familiar form, as bars of chocolate, ready to be wrapped and sold. †¢ Storing Chocolate Keep the chocolate in a cool, dry place. Chocolate is best kept at around 68 to 72 degrees Fahrenheit, the temperature of a pantry or dark cabinet. It has a shelf life of approximately one year. The normal air conditioned room provides adequate protection. Freezing chocolate is not recommended; when you freeze it and then thaw it out, it will have a greater tendency to bloom. NOTE: Bloom is the white, filmy reside that can develop on chocolate. This usually happens when the chocolate is stored in a warm place, but can happen when you freeze it. Chapter-IV: Research Methodology A)PRIMERY DATA :- As we are doing the Survey of Chocolate we have found that there many people don’t like to eat Chocolate due to various reasone and there are many people who don’t want to shift to another brand of Chocolate due to there taste. There are also some type of people who has interested in chocolate but they can not effort if bcoz of high price for it but some people for this survey they have suggested to 1. To reduce some price of chocolate 2. To make diet chocolate 3. To make spicy chocolate B)SECONDERY DATA:- Adventages :- †¢ IT makes Refreshment †¢ IT reduces calostrol by eating it 2_3 times a week †¢ IT is often classified as a non –healthy food or normally calledtunk food †¢ Dark chocolate is beneifits to the body by medical and scientific reasearcher †¢ dark chocolate may help to avoid heart disease due to presencer of antioxidants Disadventages. †¢ Chocolate is a calorie rich food with a high sugar and fat content, so regular consumption of chocolate requires reducing the caloric intake of other foods. †¢ Chocolate contains a variety of substances, some of which have addictive properties e. g sugar, theobromine and caffeine which are stimulating and mood elevating and phenethylamine which can cause endorphin releases in the brain †¢ chocolate has been linked to nervous tension as well as migraine headaches because it contains compounds known as vasoactive amines that can dilate brain vessels triggering headaches in susceptible individuals. †¢ It has high levels of arginine which is required in the replication of the herpes virus. Chocolate should be avoided by those with active or recurring herpes infections.

Effects of Vegetation on Freshwater Shrimp

Effects of Vegetation on Freshwater Shrimp The aim of this experiment was to discover whether the presence of vegetation affected the abundance of the species of freshwater shrimp Gammarus pulex. To do this an experiment was carried out involving samples taken from a variety of vegetated and non-vegetated areas. The abundance of the species in each environment was measured and compared and found to be statistically significant. Freshwater shrimp do prefer vegetated areas over non-vegetated areas due to either; increased availability of detritus, increased oxygen content or additional protection from predators. Introduction: Gammarus pulex: (freshwater shrimp) are Crustaceans that are members of the Amphipoda order. Their bodies are curved into a C shape and they average approximately 20mm in length. As seen in figure 1, Gammarus pulex have 7 pairs of legs, two of which perform a grasping function similar to humans hands whilst the other 5 are adapted to swimming. The species gills are provided with oxygen by the vibrations of their legs and the 3 pairs of structures seen on the abdomen, the gills are located on the inside of the back five legs. Gammarus pulex have a high oxygen requirements and as a result are typically found in faster flowing, oxygen rich water. They are hatched from eggs and emerge fully developed and ready to reproduce. This results in a fast rate of reproduction. The offsprings sex is determined by the temperature of the water. These shrimps mate whilst the male carries the female on its back, this will occur several times per year. Gammarus pulex Have no natural defence mechanism o ther than their camouflage and as a result they are primarily active during low sunlight periods and under shelter. As a result of Gammarus pulexs lack of a defence mechanism they are typically found under and within the aquatic fauna and rocks within rivers. Gammarus pulex are primary consumers that typically consume detritus, i.e. they consume dead and decaying matter in order to provide energy for themselves. Gammarus pulex main predators are fish which that rely on them as one of their primary sources of food, fish commonly found to consume these shrimp include: Trout, Bullheads and Salmon. Rationale: Gammarus pulex can be used as an indicator species due to their affinity for highly oxygenated water. They (Gammarus Pulex) are intolerant of polluted waters or reduced dissolved oxygen concentrations, and can therefore be used as an indicator species for water quality. A decrease in the oxygen content of an ecosystem can have serious adverse effects on the other species within the ecosystem and is one of the most common causes of Fish Kills as a result it is a variable regularly monitored by fishing agencies Due to increasing awareness of the impact humans are having on their environment and the ecosystems that we interact with, biological indicator species will become increasingly important in the identification of suitable habitats for species to be introduced to. In addition to Gammarus pulex acting as an indicator species, they also require highly oxygenated water to survive and reproduce. A lack of oxygen within the water could potentially lead to anaerobic conditions within the water. Due the fact that they require oxygen for respiration (Aerobic) this would reduce the population of the shrimp within an area. This would have a knock on effect on the secondary and tertiary consumers within the food chain that also rely on Gammarus pulex as their primary food source such as trout and salmon. As a result, the abundance of the species is of key ecological importance within these ecosystems and steps should be taken to ensure high oxygen content of the water and adequate foliage and vegetation for the species to thrive within its niche. These goals can be achieved relatively easily through the planting of vegetation for cover. This would also provide raised oxygen levels due to the respiration of the plant life to produce oxygen as a by-product . In a study by H.H Costa on investigating the affect of low concentrations of oxygen on the number of reactions per minute in Gammarus pulex, strong correlations were made between the concentration of oxygen within the water and the number of reactions taking place per minute. Low levels of oxygen would lead to decreased metabolic activity, resulting in slower growth and reproduction. As a primary consumer this would have a detrimental effect on the rest of the ecosystem as a lower population of the species would result in food being a limiting factor for fish that relied on the species as a source of food. This could also lead to the gradual decline of other primary and secondary consumers as they are used as a source of energy by the tertiary consumers to sustain themselves. Aims: The aim of the experiment was to determine whether the presence of vegetation within the water at Nettlecombe church pool would have a significant impact on the abundance of Gammarus pulex measured within an area and whether any changes in abundance could be attributed to the independent variable (presence of vegetation) by controlling or measuring the other abiotic factors within the ecosystem. Null hypothesis: Statistically, there will be no significant correlation between the abundance of freshwater shrimp and the presence of vegetation within the stream. Alternate hypothesis: Statistically, there will be significant correlation between the abundance of freshwater shrimp and the presence of vegetation within the stream. Prediction Gammarus pulex will likely show a significantly higher presence in the vegetated areas of the stream due to several key biological factors: Defense: Due to the lack of any inherent defense mechanisms, the species relies on its camouflage as its sole mechanism for defense. Naturally, high amounts of vegetation present in an area provide an aid to the protection of the freshwater shrimp from its predators and allow a greater chance of the survival of the species. Oxygen content: The oxygen within water is derived primarily from submerged plants present within the water. As these plants photosynthesize, Oxygen is released as a byproduct and dissolves into the surrounding water. This increased oxygen content would attract freshwater shrimp to an area, as they are reliant on the surrounding water for the oxygen that is necessary for them to respire aerobically. Detritus: Organic material such as decaying plant material is the primary food source of Gammarus pulex as detritivores. If vegetation is present in an area an increased amount of detritus will also be present due to the death and decay of the submerged plants. This provides an ideal food source for the Gammurus pulex and increases the chances of survival and reproduction. As a result you would expect to see an increase in the abundance of the species in areas with vegetation. Independent variable The independent variable within the experiment was the presence of a significant (+10cm) amount of vegetation submerged within the water and the lack of +10cm of vegetation present. This was varied by locating two areas, one with +10cm of submerged vegetation and another without. Dependent variable The dependent variable within the experiment was the relative abundance of the species within the two separate areas per sample. This was measured through obtaining a sample and counting the number of Gammarus pulex present per sample. Control variables Several abiotic variables were controlled within the experiment to ensure that any changes in the abundance of the freshwater shrimps could be assigned to the changing in the independent variable. In the cases in which an extraneous variable could not be controlled without compromising the integrity of the experiment, the variable was measured and any anomalous readings recorded could possibly be assigned to that abiotic factor. PH: The acidity (concentration of hydrogen ions) within the water was measured by obtaining a sample in the two areas and measuring the sample through the addition of universal indicator. The PH of the water was measured as Gammarus pulex have an aversion to water with more extreme Phs. In Responses of Gammarus pulex to modified environment II. Reactions to abnormal Hydrogen ion concentrations H.H Costa states that at the PH values of 9.6. These negative reactions can be explained through the inability of the shrimps enzymes to function at these extreme PHs. Temperature: Temperature was measured in degrees centigrade through the use of a digital thermometer that was placed in each sample, allowed to stabilize and a reading was taken. Temperature was controlled due to the reaction of Gammarus pulexs offspring to temperature, at lower temperatures, offspring within eggs will hatch as male, whilst above this they will hatch as female. This would lead to one sex becoming dominant at extremely hot or cold temperatures, which would result in a reduced abundance as the difficulty of locating a mate increased. Light: The amount of light in each sample was recorded through the use of a lux meter as close to the water as possible. This variable was measured in lux and was measured as it is likely the shrimp will be more abundant in darker areas due to the increased effectiveness of their camouflage in these areas and increased protection this offers. Depth: The depth of each location was measured through the insertion of a ruler to measure the depth of each location. The ruler was placed on the surface of the sediment to stop it being pushed too far into the soft sediment and producing an inaccurate reading. Areas were chosen as close to 10cm deep as possible to rule out the depth of the water having any significant impact on the results produced. Pilot study: Introduction: Before carrying out the main study, a pilot study was used to determine the final procedure and method of the study. This pilot study was used to identify the best sampling technique to use to collect a sufficient number of shrimps per sample. This technique had to be efficient enough to collect enough data for a statistical significance to be shown and collect the maximum number of shrimp within an area to ensure that the true abundance of the species was actually being measured. In addition, the method used to collect the shrimp had to be standardized, so that the identical technique could be carried out in different areas to ensure the reliability of the technique. This meant the same technique could be carried out again to produce as similar results as possible. In addition, the best local spot to produce enough shrimp to state a significant difference had to be located. This pilot study was used to locate the areas that would be sampled in the main experiment by locating the areas with enough vegetation to have an impact on the dependent variable. This meant looking for areas with high levels of submerged vegetation within several different rivers until enough within as Single River was found. This was to make the control of extraneous variables easier as the variation in the variables would be far larger in separate bodies of water. Within the pilot study, I also had to assess the extraneous variables that would have to be measured in my final study to prevent them interfering with the data produced. During the pilot study, I identified that the amount of light in an area seemed to be having an effect on the abundance of the species within that area. This was potentially reducing the validity of the experiment. This was because any results produced were no longer necessarily down to just the independent (presence of vegetation) variable. As a result, I included the use of a light meter in the final experiment to control and limit the impact on the data produced. Along with deciding the variables to be measured, I also had to finalize the equipment used to measure the variable in a standardized manner. The pilot study was also used to identify any potential risks or hazards within the area. Once these were identified, appropriate controls could be taken to reduce or nullify the risks from these sources. Equipment list: A 15cmx10cm Scoop net A 1 meter long ruler A digital thermometer A square 1025 cm tray that was not filled with pond water A digital stopwatch Freshwater identification sheet Pipette Method: Whilst 3 rivers were found in close proximity, only one, the church stream, contained an appropriate number of vegetated areas to non-vegetated areas close enough to carry out the experiment expediently. In addition the proximity of the locations reduced any extraneous variables that were acting on the study. Thus the church stream was used for the final experiment. 2 methods of sampling with the net were carried out, the figure of eight sample (A)and the Kick sample'(B) A) This involved locating an area to sample and standing still whilst moving the net in a figure of eight through the water. This was timed for 10 seconds and 20 seconds .This method did not disturb the sediment of the area. B) The kick sample involved placing the net downstream of the area to be sampled and kicking up the sediment into the net without moving the net. This was timed for both 10 seconds and 20 seconds using the stopwatch. After collecting each sample the contents of the net was upturned and placed into the empty tray. The tray was then filled with water from the stream. Using the identification sheet, I timed for 1 minute on the stopwatch and counted all of the Gammarus pulex present in the tray within the time. Using a pipette to move them to a separate bucket once counted A variety of sampling techniques for the control variables were then carried out in the area. The ruler was used to measure depth at a variety of locations. This provided practice at accurately measuring the depth of the water without disturbing the sediment on the bed of the stream. The digital thermometer was then placed in the water, however not placed at the bed of the stream. Risk assessment: Before carrying out the pilot and main study, a risk assessment of the risks that could pose a threat to me or those in the surrounding areas was carried out. Through this I identified the hazard (negative effect 1-10) and the risk factor (chance of the hazard actually occurring 1-10) to control this I also decided on sensible controls to minimize the chance of the hazard occurring or minimize the severity of the hazard. The risk rating was calculated by multiplying the chance of the hazard occurring with the severity of the hazard (1-100). Hazard Severity of the Hazard Chance of occurring Risk rating Control Slipping and falling. 2 6 12 Ensure the muddy parts of the bank are noted and avoided. Wear appropriate footwear to avoid tripping/slipping. Drowning in unexpected deep water. 10 1 10 Ensure all personal can swim. Identify any deep areas before beginning. Contamination from any water/aquatic life. 8 3 24 Wash hands before and after touching the water. Do not handle the aquatic life, use a pipette instead Tripping with net/ruler. 4 4 16 Wear appropriate footwear, I.e. hiking boots Discussion of results: The number of shrimp produced by using method B for 20 seconds produced the largest total number of shrimp in both vegetated areas and non-vegetated areas. In comparison, method A produced the fewest number of shrimp in both vegetated areas and non-vegetated areas. The temperatures produced seemed to be pretty constant throughout the pilot study however the two 9.8 readings were likely caused by the thermometer not being pressed to the sediment properly, reducing the reliability of those readings. The preliminary findings reflected that more shrimp were indeed found in the vegetated areas in comparison to the non-vegetated. Evaluation of pilot study: Through the use of the pilot study I was able to identify the main problems with the study and remedy them for the final study: The measuring of the temperature using the thermometer was standardized by pressing the thermometer to the bed of the stream on each measurement. The sample I chose to use was the 20 seconds kick sample This method of sampling was far superior to the figure of eight sample. This was likely to the freshwater shrimp inhabiting the upper layer of the sediment; the kick sample dislodged them into the net whereas the figure of eight did not disrupt the sediment at all resulting in only the swimming shrimp being picked up. As a result, only the number of shrimp swimming in a sampled area was being measured not the total number of shrimp in the area. I chose to use the 20 second sample as, although it was less expedient than the 10 second sample, it did provide more species and reduced the chance of anomalies cropping up through not all of the shrimp being collected. Whilst measuring the depth in step 8, I noticed that the vegetated areas where normally darker than the non- vegetated areas. To counteract this variable, I decided to control it in the main experiment by measuring the light using a lux meter. This was to ensure that the results were down to the vegetation present. During step 1 and the selection of the location of the samples, I realized that I hadnt set any specification on what classed as enough vegetation to qualify as a vegetated area. After discussing this with my tutor I decided to use 10cm measurements of submerged vegetation qualify an area as vegetated. In addition, I used areas without any vegetation for the final experiment. Whilst collecting the samples in step 4 I realized that I was disturbing the ecology further downstream on further kick samples. This was an unfortunate side effect of using the kick sample from the top of the stream down. As a result, I decided to reverse the direction for the final experiment and take the values from downstream before taking those upstream. This stopped earlier experiments influencing later ones. Main Experiment: My final experiment took into account the findings from the pilot study and used these to modify the final experiment to produce the best results I could. The changes listed above were introduced to tailor the results of the experiment into a form I could use to compare the effect of the independent variable. Intro: My final experiment consisted of 10 samples of each vegetated and non-vegetated areas being taken, with the variables light, depth, temperature, PH and number of shrimp present being measured. The revised aim of the final experiment was to measure whether the presence of vegetation affected the abundance of Gammarus pulex present and whether this difference was statistically significant. To do this I carried out a stratified sampling technique to locate areas to work in, I,e areas with +10 cm of submerged vegetation. I then used a systematic sampling technique to collect the data as I timed how many of the species I could count from a sample in 1 minute. Equipment list: A 15cmx10cm Scoop net A 1 meter long ruler A digital thermometer A square 1025 cm tray that was pre-filled with pond water A digital stopwatch Freshwater identification sheet Pipette 1 Lux meter 1 bottle of universal indicator, with a beaker to collect a sample of water A small bucket to place counted shrimp in Writing equipment and paper to record results Pipette to collect the shrimp with Method: 10 vegetated areas were first located. This involved measuring the total submerged vegetation present and ensuring it was over 10cm long. The non-vegetated areas were then located, these were areas within as close proximity to the vegetated areas as possible but without any vegetation present. Starting from downstream and moving upstream, the kick sample was performed in the first vegetated area. This involved placing the net downstream from the sampling area and kicking the sediment for 20 seconds into the net. This was timed with the stopwatch. The sample in the net was upturned into the tray. The Gammarus pulex were identified using the species identification sheet and removed from the tray and placed in the bucket using a pipette. The total number of shrimp counted in 1 minute was recorded. The contents of the tray and bucket were emptied back into the water. The depth of the sampling area was then measured using the ruler and placing it on the stream bed. The lowest value of the water rising up the ruler was measured as the depth. The thermometer was then placed lightly on the stream bed, this was left to stabilize for 10 seconds and the value was recorded The lux meter was then placed as close to the water as possible and held still. After 10 seconds the value was taken. This method was repeated for the non-vegetated areas. This procedure was carried out at all of the 10 areas. Table of results for main experiment Calculations of the means for Vegetated data: Mean PH: (7.5 x 10)/10 = 7.5 Mean temperature C: (9.8 x 2 + 9.9 x 8)/10 = 9.88 Mean Lux reading (Lux) : (240+234+194+30+13+109+603+719+560+335)/10 = 303.7 Average depth (cm) : (10.0+9.5+5.0+4.0+4.0+5.0+5.2+8.2+12.8+13.2) /10 = 7.69 Mean Shrimp (Arbitary units) : 341/10 = 34.1 Calculations of the means for Non-Vegetated data : Mean PH: (7.5 x 10)/10 = 7.5 Mean temperature C: (9.8 x 1 + 9.9 x 9)/10 = 9.89 Mean Lux reading (Lux) : (846+645+300+71+24+123+441+739+506+389)/10 = 408.4 Average depth (cm) : (12+11.5+10.5+12+8+10.5+12.4+14.5+12.4+13.3) /10 = 11.71 Mean Shrimp (Arbitary units) : 56/10 = 5.6 Anomalies: Both of the values highlighted in red, are examples of possible anomalous data that could have been caused by procedural errors. 6 is far below the average shrimp reading of 34.1, this could have been caused by the high light reading on this particular reading, reducing the shrimps camouflage and therefore their safety in this area. The value of 17 was far ahead of the mean value of 5.6 and could have been caused through the procedural error of the pipette not sucking the shrimp up and them being counted twice. These samples also could have had the random error of large amounts of sediment resulting in some shrimps not being counted or others being counted twice. The clear variation in the number of Gammarus pulex sampled in the two areas would indicate that the vegetation did have an effect on the abundance of the population. Likely due to the increased defense offered by these areas and also how much easier it would be to obtain decomposing plant matter (Detritus) as a food source. The largest value for a vegetated shrimp was 79, with the lowest being 6. This is in comparison to the highest value for non-vegetated areas being 17, with the lowest being 2. The similarities between the abiotic factors, primarily temperature and P.H also increase the validity of the results produced. We can be sure that the results are actually as a result of the presence of the vegetation. It should be noted that none of the samples contained no freshwater shrimp, leading to the assumption that the water likely has a high oxygen content and has few pollutants. This is backed up by the location of Nettlecombe, a relatively rural area with few large roads runnin g nearby. Statistics: Within this study I decided to use the Mann Whitney U test to ascertain whether the difference between the two values was enough to reject the null hypothesis and convey a significant difference between the two sets of data. Null Hypothesis: There will be no significant statistical difference between the data drawn from vegetated samples and non-vegetated samples in the Nettlecombe church stream on the 14/10/12 The lowest U value calculated was 6, The critical value at 0.1 is 16 meaning that the Null Hypothesis can be rejected at the 0.1 significance level This allows us to accept the alternate hypothesis, and although there were small overlaps in the data, the difference between the two sets of data values is significant. Evaluation: Throughout the experiment, every effort was made to control or measure extraneous variables that could have an effect on the results of the experiment. As a result, the validity of the experiment is quite high; the values for all the abiotic variables were measured and have similar values for both the Vegetated areas and the non-vegetated areas. As a result we can make the assumption that any changes in the dependent variable, were in fact down to the independent variable. The pilot study was carried out to aid in the identification of any confounding variables, risks or extraneous variables that could potentially impact on the validity of the results produced and as a result of the pilot study, controls were put in place to counteract these variables. In addition, due to the standardized methodology carried out within both the pilot experiment and the final experiment, the method could be repeated under similar conditions to produce similar results. Resulting in a high reliability of the data. As the Mann Whitney U test allowed us to reject the null hypothesis at the 0.01% significance level, we must assume that the results produced show a significant change between the two environments. Although the results proved to be statistically significant, several procedural errors should be considered. Even with a 20 second kick sample, not all of the shrimp in a certain area could be collected as the shrimp likely inhabit different layers of the sediment. The speed at which the shrimp were counted at could also vary considerably based on the amount of sediment present in a sample, in a sample with high amounts of sediment, it would take longer to count and remove all of the shrimp within a minute than it would be in a clear sample. To counteract this, a stratified sample could be put in place; all of the shrimp from a sample could be counted rather than just counting for one minute. Systematic errors within parts of the equipment could result in repeatedly wrong measurements, e.g the Lux meter may not be calibrated properly and give readings that are continually 50 higher than they should be. Percentage error can also become a problem and whilst small, can be noticeable when accounted for in multiple datasets e.g. 0.01/500 x100 = 0.002 % percentage error There are also several ethical considerations to take into account when using live organisms within experiments. For instance the ecosystem is being massively disrupted through the use of a kick sample. All of the layers of sediment are being removed and many organisms are being collected in a sample that didnt have to be. A more specific and less environmentally invasive procedure could instead be carried out using the figure of eight sampling method so as not to disturb the stream bed ecosystem as much. Conclusion From the data and evidence provided above, a reliable argument can be put forward that the species Gammarus pulex does favor vegetated conditions over non-vegetated conditions. This could be down to a variety of reasons: Gammarus pulex is an aerobic organism and thus favors oxygenated conditions. During photosynthesis in submerged plants, oxygen is produced as a byproduct which diffuses into the surrounding environment (the water) this results in increased oxygen content. The oxygen content at this point is not acting as a limiting factor in the population of Gammarus pulux and thus an increase in the activity within the reproductive cycle of the species. This is demonstrated in the image to the right. Gammarus pulex favors the vegetated environment due to the increased source of detritus present through the decomposition of the plants present in the environment. This increased source of food and energy means the species expends more energy within its reproductive cycle and fewer of the species die due to starvation. Thus the population of the species in these conditions increases. Gammarus pulex could also potentially favor the vegetated environment due to the increased camouflage and protection it provides it. This could be part of a behavioral adaptation the organism has developed to protect itself from the fish that hunt it. This would result in large numbers gathering in the areas that they were safe and fewer of them being consumed by secondary and tertiary consumers resulting in larger numbers of them in the vegetated areas. Source evaluation Within my experiment, I extensively referenced and used H.H Costas work into the differing effects of modified environments on Gammarus pulex. This work was published in the scientific, peer reviewed journal Crustaceana. This journal is is a leading journal in the world on crustacean research. As a result I feel the peer reviewed work is both reliable and accurate and although published in 1967, the more recent research (Field guide to the water life of Britain 1984) also agrees with the core principles described in H.H Costas work. In addition to several peer reviewed journals, one of my sources was an Information pack produced by the University of Florida, a well respected academic institute unlikely to publish inaccurate or unreliable information. As well as the paper sources listed, I used a variety of websites to gather data on various aquatic processes and mechanisms. Whilst I trust the majority of the websites, certain websites such as source 2, dont back up many of the claims with any real evidence which could be a sign of unreliable or inaccurate information. Further research Further research could be carried out into the preferences of Gammarus pulex through an experiment designed to ascertain the type of vegetation that they prefer and also the nutritional value of the varying types of detritus to the organism. In addition, research could be done into pinpointing the exact reason that the species prefers to reside within vegetative conditions rather than non-vegetative.